Method Development and Validation of RP-HPLC for the Simultaneous Estimation of Niacin and Trans-Resveratrol in Bulk Drug and Dosage Forms by Using Human Plasma

In this project, the study includes the method development and validation of Niacin and Resveratrol in bio-analytical method using RP-HPLC. The Niacin and Resveratrol were journalised individually. No combined method has been found so far. This method was very accurate, precise, robust and simple when compared to other methods. The study has been summarised below: The introduction explain about the Nutraceutical drugs developed in Bio-analytical method. As the Bio- analytical methods shows the Bioavailability of the drug present and reacted in our body. The method has been developed and optimized according to the ICH Q2 (R1) guidelines. Literature survey has been reviewed with many techniques and they provided different results. Clinical study were also published and that to were helped in providing information. The drugs are developed combinedly for our optimised study. The Aim and Plan of work defines about the project. According to the drug profile, Proper selection of the method depends upon the nature of the sample, its molecular weight and solubility. The drugs selected in the present study are polar in nature and hence RP-HPLC method was used because of its simplicity and suitability. Materials and Methods briefs out the preparation of standard and internal standard drugs, sample of dosage forms which are optimized. As the physical properties are same for Caffiene (IS) as that of the standard drugs. So, it was selected as the Internal standard. The results (tables, graphs) were shown for the conclusion of our study which are developed and validated as per ICH guidelines: UV spectrum was ranged between 200-400 nm. 214 and 306 nm showed maximum absorbance for Niacin and Resveratrol respectively. The 270 nm was found as the isobestic point for these two drugs. With Phenomenex luna C18 column the drug was not eluted. No peaks were obtained. The column was changed to Sunfire C18, initially methanol was used which gave high pressure which is beyond the limit. The linearity was found to be 3.6-25.2;g/ml, 1-7;g/ml and correlation coefficient was found to be 0.999, 0.998 for Niacin and Resveratrol respectively. The retention time was found to be 1.8, 2.6 and 5.1 mins for Niacin, Caffeine and Resveratrol respectively. The accuracy and precision was found to be 98-99%. The recovery were found to be 76-78%. The LOD and LOQ was found to be 0.05, 0.03 for Niacin and 0.15, 0.10 for Resveratrol. The short term stability study was found to be 97.50-98.86%. The post preparative stability study was found to be 98.02-99.28% The freeze and thaw cycles was found to be 97.92-100.41%. The result of dosage form analysis by developed method was compatible with the plasma. There was no interference of excipient for analysis of dosage forms. The developed method can be applied for the studies of pharmacokinetics parameters of Anti- cholesterol drugs for Niacin and Resveratrol and its metabolites in human plasma. It can also be applied in therapeutic drug monitoring practices and clinical toxicological assays. CONCLUSION: An isocratic novel method has been developed for simultaneous estimation of Niacin and Resveratrol in plasma by RP-HPLC coupled with PDA detector. The proposed method has the ability to separate the components within 10 minutes. This method is simple, rapid and time consuming when compared to other methods so it offers a significant advantage in short run time, economic, rapid extraction, easy sample preparation, good recovery and wide linearity range. All parameters such as precision, accuracy, linearity, robustness, LOD, LOQ and system suitability was validated as per ICH guidelines and the result shows that the method was reliable and acceptable. Therefore, we can conclude that the proposed chromatographic method can be used to analyze samples in routine assay of Niacin and Resveratrol in Bulk drugs and in Plasma

An In-Depth Analysis on Efficiency and Vulnerabilities on a Cloud-Based Searchable Symmetric Encryption Solution

Searchable Symmetric Encryption (SSE) has come to be as an integral cryptographic approach in a world where digital privacy is essential. The capacity to search through encrypted data whilst maintaining its integrity meets the most important demand for security and confidentiality in a society that is increasingly dependent on cloud-based services and data storage. SSE offers efficient processing of queries over encrypted datasets, allowing entities to comply with data privacy rules while preserving database usability. Our research goes into this need, concentrating on the development and thorough testing of an SSE system based on Curtmola’s architecture and employing Advanced Encryption Standard (AES) in Cypher Block Chaining (CBC) mode. A primary goal of the research is to conduct a thorough evaluation of the security and performance of the system. In order to assess search performance, a variety of database settings were extensively tested, and the system's security was tested by simulating intricate threat scenarios such as count attacks and leakage abuse. The efficiency of operation and cryptographic robustness of the SSE system are critically examined by these reviews

Method Development and Validation of RP-HPLC for the Simultaneous Estimation of Niacin and Trans-Resveratrol in Bulk Drug and Dosage Forms by Using Human Plasma

An isocratic novel method has been developed for simultaneous estimation of Niacin and Resveratrol in plasma by RP-HPLC coupled with PDA detector. The proposed method has the ability to separate the components within 10 minutes. This method is simple, rapid and time consuming when compared to other methods so it offers a significant advantage in short run time, economic, rapid extraction, easy sample preparation, good recovery and wide linearity range. All parameters such as precision, accuracy, linearity, robustness, LOD, LOQ and system suitability was validated as per ICH guidelines and the result shows that the method was reliable and acceptable. Therefore, we can conclude that the proposed chromatographic method can be used to analyze samples in routine assay of Niacin and Resveratrol in Bulk drugs and in Plasma

A Survey on the application of Data Science And Analytics in the field of Organised Sports

The application of Data Science and Analytics to optimize or predict outcomes is Ubiquitous in the Modern World. Data Science and Analytics have optimized almost every domain that exists in the market. In our survey, we focus on how the field of Analytics has been adopted in the field of sports, and how it has contributed to the transformation of the game right from the assessment of on-field players and their selection to the prediction of winning team and to the marketing of tickets and business aspects of big sports tournaments. We will present the analytical tools, algorithms, and methodologies adopted in the field of Sports Analytics for different sports and also present our views on the same and we will also compare and contrast these existing approaches. By doing so, we will also present the best tools, algorithms, and analytical methodologies to be considered by anyone who is looking to experiment with sports data and analyze various aspects of the game.Comment: arXiv admin note: substantial text overlap with arXiv:2209.0699

Hysteresis Loop Effect on Coronal Index and Solar Flare Index During Solar Cycles 22 and 23

The hysteresis loop effect on the solar activity as coronal index (CI) and solar flare index (SFI) over cosmic ray intensity (CRI) at the low cut-off rigidities neutron monitoring station Oulu on the monthly basis data for the period January 1, 1986 to December 31, 2008 has been studied. It is observed that the shape of the hysteresis loops is varying from solar cycle 22 to 23. The hysteresis loops for solar cycle 23 are broad ovals whereas for solar cycle 22 are nearly flat. A complementary feature of the cosmic ray intensity decreases more rapidly as the increase in solar activity (coronal index and solar flare index). The positive solar polar magnetic parameter (A>0) and negative solar polar magnetic parameter (A<0) is representing the hysteresis loops

Cultural basis of social ‘deficits’ in autism spectrum disorders

There is very little research that specifically looks at how autism spectrum disorders are perceived in various communities. This qualitative research was conducted with parents who had children on the autistic spectrum belonging to four different ethnic communities (White British, Somali, West African and South Asian- 63 in total) and living in the UK. The study found that the importance that the parents give to various social skills varied on the basis of their cultural background and the gender of the parent. This is an important aspect to consider while providing support and services to individuals on the autism spectrum and their family members if the services have to be appropriate for their needs. This consideration would also enable the individuals on the autism spectrum to develop appropriate social skills required within their cultural groups. This is a preliminary study and further research on the topic is required

Supersymmetric States in Large N Chern-Simons-Matter Theories

In this paper we study the spectrum of BPS operators/states in N=2 superconformal U(N) Chern-Simons-matter theories with adjoint chiral matter fields, with and without superpotential. The superconformal indices and conjectures on the full supersymmetric spectrum of the theories in the large N limit with up to two adjoint matter fields are presented. Our results suggest that some of these theories may have supergravity duals at strong coupling, while some others may be dual to higher spin theories of gravity at strong coupling. For the N=2 theory with no superpotential, we study the renormalization of R-charge at finite 't Hooft coupling using "Z-minimization". A particularly intriguing result is found in the case of one adjoint matter.Comment: 53 pages, 18 figures. v2: improved numerics, additional comments adde

Radiation tolerance of two-dimensional material-based devices for space applications

Characteristic for devices based on two-dimensional materials are their low size, weight and power requirements. This makes them advantageous for use in space instrumentation, including photovoltaics, batteries, electronics, sensors and light sources for long-distance quantum communication. Here, we present for the first time a comprehensive study on combined radiation effects in earth's atmosphere on various devices based on these nanomaterials. Using theoretical modeling packages, we estimate relevant radiation levels and then expose field-effect transistors, single-photon sources and monolayers as building blocks for future electronics to gamma-rays, protons and electrons. The devices show negligible change in performance after the irradiation, suggesting robust suitability for space use. Under excessive $\gamma$-radiation, however, monolayer WS$_2$ showed decreased defect densities, identified by an increase in photoluminescence, carrier lifetime and a change in doping ratio proportional to the photon flux. The underlying mechanism was traced back to radiation-induced defect healing, wherein dissociated oxygen passivates sulfur vacancies

Entangled Dilaton Dyons

Einstein-Maxwell theory coupled to a dilaton is known to give rise to extremal solutions with hyperscaling violation. We study the behaviour of these solutions in the presence of a small magnetic field. We find that in a region of parameter space the magnetic field is relevant in the infra-red and completely changes the behaviour of the solution which now flows to an $AdS_2\times R^2$ attractor. As a result there is an extensive ground state entropy and the entanglement entropy of a sufficiently big region on the boundary grows like the volume. In particular, this happens for values of parameters at which the purely electric theory has an entanglement entropy growing with the area, $A$, like $A \log(A)$ which is believed to be a characteristic feature of a Fermi surface. Some other thermodynamic properties are also analysed and a more detailed characterisation of the entanglement entropy is also carried out in the presence of a magnetic field. Other regions of parameter space not described by the $AdS_2\times R^2$ end point are also discussed.Comment: Some comments regarding comparison with weakly coupled Fermi liquid changed, typos corrected and caption of a figure modifie